Christopher MutungiOnyango CalvinJaros DorisHenle ThomasRohm Harald2024-07-112009-07-13Mutungi, C., Onyango, C., Jaros, D., Henle, T., & Rohm, H. (2009). Determination of optimum conditions for enzymatic debranching of cassava starch and synthesis of resistant starch type III using central composite rotatable design. Starch‐Stärke, 61(7), 367-376.https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=Determination+of+Optimum+Conditions+for+Enzymatic+Debranching+of+Cassava+Starch+and++Synthesis+of+Resistant+Starch+Type+III+using+Central+Composite+Rotatable+Design+&btnG=#d=gs_cit&t=1720677633415&u=%2Fscholar%3Fq%3Dinfo%3AHplwsXt8fMQJ%3Ascholar.google.com%2F%26output%3Dcite%26scirp%3D0%26hl%3Denhttps://repository.nrf.go.ke/handle/123456789/1293Full-text ArticleCassava starch was debranched by treatment with isoamylase and pullulanase and the yield of resistant starch type III (RS III) optimized with respect to starch solids concentration (7.5-15%, w/v), incubation time (8-24 h) and enzyme concentration using central composite rotatable design. Higher concentrations of pullulanase (10-35 U/g starch) compared to isoamylase (30–90 mU/g starch) were required to give a similar degree of starch hydrolysis within the experimental domain. A clear debranching end-point was identifiable by following the reducing value, blue value and β-hydrolysis limit of cassava starches debranched using isoamylase. It was difficult to define a debranching endpoint of pullulanase treatment by these parameters due to contaminating α-D-(1→4) activity. The yield of RS III was significantly higher in isoamylolysates and increased steadily with increasing degree of hydrolysis to peak at 57.3%. Purification of the debranched material further increased the RS III yield to 64.1%. Prolonged (24 h) hydrolysis of cassava starch with high concentration of pullulanase (35 U/g) gave lower RS III contents in the purified (34.2%) and unpurified (36.2%) hydrolysates compared to 49.5 and 62.4%, respectively, at moderate pullulanase concentration (22.5 U/g) and incubation time (16 h).enResistant StarchCassavaStarchEnzymatic DebranchingCentral Composite Rotatable DesignStarch ModificationArticle