Browsing by Author "Gatebe Erastus"

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    Effect of Biological Control Antagonists Adsorbed on Chitosan Immobilized Silica Nanocomposite on Ralstonia solanacearum and Growth of Tomato Seedlings
    (SCIENCEDOMAIN international, 2016-01-11) Gatahi Dennis; Wanyika Harrison; Kihurani Agnes; Gatebe Erastus
    Background: Biological control holds promise in managing bacterial wilt disease. However, its efficacy is limited by harsh environmental conditions when applied without use of suitable carrier materials. Aim: The study entailed synthesis of nanocarrier materials for biological control agents (BCAs) using Chitosan and silica nanocomposites. Site and Duration: The experiments were carried out at Jomo Kenyatta University of Agriculture and Technology for a period of two years June 2013 to June 2015. Methodology: The experiments were conducted using a completely randomized design with three replications. Deacetylation, functionalization and immobilization of chitin on mesoporous silica nanoparticles (MSN) to form chitosan immobilized silica nanocomposites (CISNC) gel was done. Results: This resulted in formation of chitosan nanoparticles and CISNC with crystallite sizes of 2.8 and 4.4 nm respectively. BCAs were adsorbed on CISNC gel. Characterization of the bio- nanocomposites showed that they had physisorption properties thus, ideal carriers for BCAs. CISNC gel had the highest significant (P=.05) sorption properties with 75% and 65% adsorption and desorption respectively of BCAs. Efficacy trials were done by in vitro pathogen inhibition and greenhouse bioassays using tomato seedlings. Adsorption of BCAs on CISNC gel significantly (P=.05) increased inhibition efficacy of BCAs on R. solanacearum from 50 to 70%. This was attributed to the antibacterial effect of the individual substances and the overall synergy acquired. Further, BCA-CISNC gel forms a film around root hairs, initiates fast wound healing mechanism and induce prophylactic effect on tomato seedlings challenged with R. solanacearum pathogen, decreasing wilting incidences from 45 to 25%. Additionally, BCA-CISNC complex significantly (P=.05) increased tomato seed germination from 70 to 80% and growth rate from 12 to 15% due to enhanced water utilization efficiency, induced phytohormones and nutritional benefit. BCAs also aided faster nutrient release, absorption and utilization by tomato plants. Conclusion: Therefore, adsorption of bacterial, fungal and phage biocontrol agents on CISNC gel, a complex hitherto not reported to have been used in R. solanacearum disease control, enhanced microbial efficacy against the pathogen and increased tomato productivity.