Browsing by Author "Mwaura, Francis"

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  • Publication
    Publication
    Isolation of Actinomycetes from Geothermal Vents of Menengai Crater in Kenya
    (International Journal of Molecular Biology, 2017-07-20) Waithaka, Paul Njenga; Mwaura, Francis; Wagacha, John M.; Gathuru, Eliud Mugu
    The current study was carried out to isolate actinomycetes from the geothermal vents of Menengai crater. Soil samples were collected from vents A and D in sterile polythene papers and transported to Egerton University, Department of biological sciences laboratories. The samples were air dried on the benches for one week. To kill vegetative bacteria, the soil samples were heat in a hot air oven for 1h before serial dilution to 10-6. The samples separately plated on Starch casein agar, Luria Bertani agar and starch nitrate agar in which nystatin and nalidixic acid had been added to reduce the growth of fungi and other types of bacteria. Incubation was carried out at 30 o C for up to a period of one Month. The isolated actinomycetes were characterized by cultural, morphological and biochemical means. There was no significant difference in the number of actinomycetes isolated between vents A and vents D(P=0.439). However, the number of actinomycetes isolated using the three isolation media varied significantly (F=37, P=0.03). Totally, 16 actinomycetes were isolated from the vents. It is recommended that the isolates be tested for antagonism against pathogenic microorganism.
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    Publication
    Wildlife Population Change in Africa from the Eyes of the Public—The Case of Mara Enoonkishu Conservancy in Southern Kenya
    (Scientific Research Publishing, 2016-06) Masiga, Dorothy Syallow; Mwaura, Francis; Mukhovi, Mikalitsa S.
    The aims of the study were to: 1) determine the wildlife population trends in the Mara Enoonkishu Conservancy from the eyes and minds of the public, 2) assess the recent trends in wildlife populations based on actual scientific counts, and 3) compare the two patterns and establish whether they are similar or not. The solicitation of public views was based on household surveys using a standard questionnaire which was administered to 115 randomly selected respondents. The dominant wildlife species according to 39% of the respondents was the elephant followed by the zebra with 35%. 40% of the respondents indicated that the rhino population had declined followed by wild dogs (7%) and cheetah (6%). The actual counts showed that the herbivores with increasing populations included the rhino (r2 = 0.9992), impala (r2 = 0.9824), Thomson’s gazelle (r2 = 0.6965), Grant’s gazelle (r2 = 0.7052), elephant (r2 = 0.5798), and topi (r2 = 0.4426) while the increasing predators included the lion (r2 = 1.000), cheetah (r2 = 0.9093) and hyena (r2 = 0.8462). The actual wildlife counts appeared to tally with the public views only for the elephant and zebra. The actual counts contradicted the public views in terms of the rhino, lion and gazelles whose numbers were on the increase but the people did not appear to notice. Based on this, it was concluded that the public view on the wildlife population trend was not similar to the actual wildlife population status on the ground based on scientific monitoring