Browsing by Author "Obonyo Meshack Amos"

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    Modulation of Aflatoxin Production by Interaction of Aspergillus Species from Eastern Kenya
    (IOSR Journal of Environmental Science, 2019-04-13) Lagat Micah Kipkemboi; Toroitich Faith Jebet; Obonyo Meshack Amos; Makonde Huxley Mae
    Aflatoxin contamination of grain has continued to pose a significant threat to sustainable food security and trade worldwide. In the field, there are incidences of varying contamination levels in grain within the same niche. We hypothesize that the variation could be due to fungal species interaction at the kernel level. Seventeen isolates (14 Aspergillus flavus and 3 Aspergillus parasiticus) from Eastern Kenya were selected and confirmed for toxigenicity using Dichlorvos-Ammonia method, then cultured based on their isolation frequencies and co-existence in nature. The fungi were co-cultured using maize kernels as growth substrate, which was then used to estimate aflatoxin produced in a competitive ELISA. A one-sample two-tailed t-test was carried out to determine the degree of significance in aflatoxin production. Eight isolates were non-toxigenic, while nine were toxigenic. When co-cultured with some non-toxigenic isolates such as A. parasiticus (2EM0601), the most toxigenic A. flavus isolate (1EM1901) significantly increased aflatoxin production, while it reduced with others. These observations warrant investigation on the interaction of Aspergillus species in culture especially given their diverse toxigenic potential. We concluded that colony-mediated aflatoxin production could explain the variations of toxin levels observed in freshly collected field samples.