Browsing by Author "Okumu Oliver Otieno"

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    Effect of Lablab Green Manure on Population of Soil Microorganisms and Establishment of Common Bean (Phaseolus vulgaris L.)
    (American Journal of Agricultural Science, 2018-11-05) Okumu Oliver Otieno; Muthomi James; Ojiem John; Narla Rama; Nderitu John
    Green manures improve soil health and fertility but application of undecomposed lablab residues lead to low crop establishment. The study was carried out to determine the effect of green manure on microbial population and establishment of bean crop. Bean varieties were planted on plots each treated with lablab green manure at one ton ha-1 over whole plots and in rows, DAP fertilizer was applied at 75 kg/ha. Data was collected on microbial population, crop emergence, root rot incidence and severity, and yield. Green manure incorporation increased soil organic carbon, nitrogen, phosphorus and potassium but reduced germination percentage by about 35% and increased incidences of root rot by 30% compared to plots without green manure. The population of root rot pathogens was significantly higher in plots treated with green manure two weeks after emergence while the population of saprophytic fungi was low. Plots treated with lablab green manure reduced grain and biomass yields by 25%. Green manure increases soil nutrients directly and improves crop establishment after decomposition. Results of the study revealed a considerable increase in the population of root rot pathogens with corresponding decrease in the population of antagonistic fungi thus the poor emergence and crop establishment can be associated with increase in population of root rot pathogens and stress experienced by seeds during decomposition.
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    Effect of Legume Extracts on Germination, Seedling Health of Beans (Phaseolus vulgaris L.) and Soil Microorganisms
    (International Journal of Plant & Soil Science, 2019-05-10) Okumu Oliver Otieno; Muthomi James Wanjohi; Ojiem John; Narla Rama; Nderitu John Huria
    Application of undecomposed green manure has been reported to cause poor emergence and establishment of common beans in the field. Therefore, to understand the mechanisms’ contributing to the poor crop establishment, the effect of extracts from fresh and decomposed legume green manures on bean seed germination, fungal mycelial growth, spore germination and germ tube elongation were evaluated. The extracts were prepared in either ethanol or distilled water. Data was collected on percentage seed germination, seedling length, mycelial radial growth, spore germination and germ tube elongation. Ethanol extracts from fresh lablab inhibited bean germination by 56%, increased mean germination time to 8 days, and decreased germination index while ethanol extracts of groundnut and beans caused highest inhibition in bean shoot length and reduced biomass. Ethanol extracts from fresh green manures significantly inhibited fungal mycelia growth while the aqueous extracts from beans, groundnuts and soybean had significant level of antifungal activity while aqueous lablab extracts stimulated mycelial. Aqueous extract of lablab and soybean enhanced spore germination by over 70% with more pronounced effect on germ tube length and number of germ tubes by 8.0% and 13% respectively. The study comparatively reveals that the extract of lablab was inhibitory to common bean germination compared to other legume extracts and also stimulated the growth of root rot pathogens that may have resulted in poor establishment of beans.
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    Occurrence of Fusarium Head Blight of Wheat and Associated Mycotoxins in Narok and Nakuru Counties, Kenya
    (Science and Eduacation Publishing, 2016) Wagacha John Maina; Njeru Nancy Karimi; Okumu Oliver Otieno; Muthomi James Wanjohi; Mutegi Charity Kawira
    Fusarium head blight (FHB) is an important disease of small grain cereals. This study assessed the incidence and severity of FHB of wheat at hard dough stage, and levels of deoxynivalenol and T2-toxin at harvest by direct competitive enzyme linked immuno-sorbent assay. Wheat ears were randomly sampled from 51 farms in Narok County and 51 farms in Nakuru County at hard dough stage while wheat kernels were sampled at harvest. Prevalence of FHB in both Counties was 100%. The mean incidence of FHB was 28.4% and 20.5% in Narok and Nakuru Counties, respectively with 16.9% and 11.7% corresponding severity. Over 14 Fusarium spp. were isolated from wheat ears and kernels with F. avenaceum, F. poae and F. graminearum being isolated in the highest incidence. Levels of DON in the kernels ranged from below limit of detection n (
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    Residual Effects of Lablab Green Manure on Root Rot Pathogens and Performance of Common Bean (Phaseolus Vulgaris L.)
    (Innovations in Agriculture, 2023-10-04) Okumu Oliver Otieno; Muthomi James Wanjohi; Ojiem John; Nderitu John Huria
    Organic manures often have considerable residual effect on the subsequent crops in the soil. Residual effects of lablab green manure on root rot pathogens and bean crop establishment was evaluated by conducting field experiments at two sites with varying soil fertility in Nandi South. Common bean varieties KK8 and GLP2 seeds were planted on plots previously treated with different soil amendments. Soil samples were collected before planting and six weeks after emergence to determine the populations of root rot pathogens. Throughout the experiment, data was collected on crop emergence, plant stand, root rot incidence and severity, as well as crop yield. There was increase in crop emergence by 40% for GLP2 and 19% for KK8 with corresponding 7% reduction in root rot incidence in plots previously treated with lablab green manure. There were variations in the population of root rot pathogens between the two studies, with Kapkerer exhibiting a higher pathogen population compared to Koibem. The primary root rot pathogens isolated and identified were F. solani, F. oxysporum and Pythium ultimum, F. solani and F. oxysporum were the most dominant species isolated in over 90% plant and soil samples. Residual plots treated with lablab green manure recorded a substantial in yield with an impressive increase of 22% when compared to other treatments. Correlation analysis established that disease incidence, and severity was negatively correlated with yield while plant stand was positively correlated with yield. Our research highlights the long-term benefits of lablab green manure as an organic soil amendment, showcasing its ability to improve crop emergence, reduce root rot incidence, and boost overall crop yield. This knowledge can guide farmers in optimizing their agricultural practices for increased productivity and sustainable soil health.