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Enzymatic activity and brine shrimp lethality of venom from the large brown spitting cobra (Naja ashei) and its neutralization by antivenom

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dc.contributor.authorMitchel Otieno Okumu, James Mucunu Mbaria, Joseph Kangangi Gikunju, Paul Gichohi Mbuthia, Vincent Odongo Madadi and Francis Okumu Ochola
dc.date.accessioned2025-07-16T07:24:16Z
dc.date.issued2020
dc.description.abstractNaja ashei is a snake of medical importance in Kenya, Ethiopia, Somalia, Uganda, and Tanzania. Little is known about the enzymatic (snake venom phospholipases A2; svPLA2’s) and toxic (lethal) activities of N. ashei venom and crucially, the safety and capacity of available antivenom to neutralize these effects. This study aimed to determine the enzymatic and toxic activities of N. ashei venom and the capacity of Indian and Mexican manufactured antivenoms to neutralize these effects. The protein content of the venom and the test antivenoms were also evaluated. A 12-point log concentration–response curve (0.5–22.5 µg/mL) was generated on an agarose-egg yolk model to predict the svPLA2 activity of the venom. The toxicity profiles of the venom and antivenoms were evaluated in the brine shrimp lethality assay. Lowry’s method was used for protein estimation.
dc.description.sponsorshipNRF
dc.identifier.urihttps://doi.org/10.1186/s13104-020-05167-2
dc.identifier.urihttps://repository.nrf.go.ke/handle/123456789/1430
dc.language.isoen
dc.publisherBMC Research Notes
dc.relation.ispartofseriesOkumu et al. BMC Res Notes 2020 325
dc.subjectSnake venom phospholipases A2
dc.subjectBrine shrimp lethality assay
dc.subjectSnake venom toxicity
dc.subjectNaja ashei
dc.subjectBrine shrimp
dc.subjectArtemia salina
dc.subjectProbit analysis
dc.subjectLC50
dc.subjectEC50
dc.subjectMPC50
dc.titleEnzymatic activity and brine shrimp lethality of venom from the large brown spitting cobra (Naja ashei) and its neutralization by antivenom
dc.typeArticle
dspace.entity.typePublication

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