Publication: IN-VITRO CYTOTOXICITY OF THREE SELECTED MEDICINAL PLANT EXTRACTS FROM KENYA.
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2017-05-17
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WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES
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Abstract
In Kenya, medicinal plants have been used by the local people as
traditional medicine to treat different human ailments from time
immemorial. However, safety of most of these plants has not been
determined hence the present study seeks to determine the in vitro
cytotoxicity of the three medicinal plants namely; Ehretia cymosa
Thonn, Dovyalis abyssinica (A. Rich.) Warb. and Periplocca
linearifolia Dill & A. Rich that are commonly used to treat various
diseases. The ethnobotanical survey was carried out using a structured
questionnaire. Fresh plants were collected from the field and air dried
under shade at 25oC and later ground into powder and extracted using
acetone and water. In vitro cytotoxicity test was carried out following a modified rapid
calorimetric assay using actively dividing sub-confluent Vero E6 cells. Cell toxicity showed
that most of the plant extracts tested were not cytotoxic having a CC50(µg/ml) of ≥100 against
Vero cell lines except the acetone extracts of P. linearifolia that were moderately toxic with a
CC50(µg/ml) of 12.5, suggesting former extracts may be safe as antimicrobials.