Publication:
Strategies for Improving Hydrolytic Efficiency of Crude Multienzyme Extracts in Mushroom Processing

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dc.contributor.authorOjwang D. Otieno
dc.contributor.authorMulaa F. Jakim
dc.contributor.authorObiero George
dc.contributor.authorMidiwo Jacob
dc.date.accessioned2024-07-10T21:30:07Z
dc.date.issued2022-11
dc.descriptionArticle
dc.description.abstractThe current study investigated and optimized key process parameters affecting mushroom hydrolysis with crude enzymatic extract. The crude enzyme was prepared by solid-state fermentation of pineapple peels using Aspergillus niger. The reaction parameters viz. time, temperature, pH and enzyme concentration were optimized using the central composite design of the response surface methodology. The model predicted glucose yield of 1.49 mg/mL at optimal pH of 6.5, temperature of 50 °C, enzyme loading of 5 % (v/v), and reaction time of 12 h. Mushroom hydrolysis at the same optimal model conditions, increased glucose yield by 10%. More so supplementing SSF media with 0.2% (w/v) Tween-80 and 0.08% (w/v) yeast extract at moisture level of 70–75% significantly (p value < 0.05) improved hydrolytic efficiency of the crude enzyme extract by 2.2-fold. This study provides baseline data that will be useful in developing a low-cost enzyme-based process for hydrolyzing mushrooms to recover high-value products.
dc.identifier.citationOtieno, O. D., Jakim, M. F., George, O., & Jacob, M. (2022). Strategies for improving hydrolytic efficiency of crude multienzyme extracts in mushroom processing. Heliyon, 8(11).
dc.identifier.urihttps://www.cell.com/heliyon/fulltext/S2405-8440(22)02600-7
dc.identifier.urihttps://repository.nrf.go.ke/handle/123456789/1275
dc.language.isoen
dc.publisherElsevier Inc
dc.subjectBioprocessing
dc.subjectCrude enzymes
dc.subjectMushrooms
dc.subjectResponse surface methodology
dc.titleStrategies for Improving Hydrolytic Efficiency of Crude Multienzyme Extracts in Mushroom Processing
dc.typeArticle
dspace.entity.typePublication

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