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PfHRP2-PfHRP3 diversity among Kenyan isolates and comparative evaluation of PfHRP2/pLDH malaria RDT with microscopy and nested PCR methodologies

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dc.contributor.authorNderu, David
dc.contributor.authorKimani, Francis
dc.contributor.authorThiong'o, Kelvin
dc.contributor.authorAkinyi, Maureen
dc.contributor.authorKaranja, Evaline
dc.contributor.authorMeyer, Christian G.
dc.contributor.authorVelavan, Thirumalaisamy P.
dc.date.accessioned2024-06-19T17:31:19Z
dc.date.issued2018-12-01
dc.description.abstractRapid diagnostic tests (RDT) are valuable tools that support prudent and timely use of antimalarial drugs, particularly if reliable microscopy is not available. However, the performance and reliability of these tests vary between and within geographical regions. The present study evaluated the performance of routine malaria RDT in Kenyan febrile patients in Busia County, Kenya. A cross sectional study design was employed to recruit febrile patients attending health facilities between August and November 2016. A total of 192 febrile patients who were slide positive and negative were evaluated for their infection status by nested PCR and RDTs (PfHRP2/pLDH). In addition, P. falciparum diversity of the histidine-rich proteins 2 and 3, that influences the RDT test results were determined. All individuals were P. falciparum positive. Among the investigated 192 febrile patients, 76 (40%) were positive by microscopy, 101 (53%) by RDTs and 80 (42%) were PCR positive. The performance of the CareStartâ„¢ HRP2/pLDH (pf) RDTs was better than microscopy (Sensitivity 94%; Specificity 75%) and Nucleic acid testing (sensitivity 95%, specificity 77%) with high negative predictive values, indicating the suitability of the RDT in routine practice. Specific pfhrp2/pfhrp3 deletions shown to associate with RDT false negativity was not observed. However, high genetic diversity among pfhrp2 gene was observed. Eleven new PfHRP2 and nine PfHRP3 repeats were observed. False positivity by microscopy and under reporting of infections may thus be a barrier in malaria control and elimination programs. The HRP2/pLDH(Pf) based RDT yet demonstrate to be an effective tool for malaria surveillance program.
dc.description.sponsorshipInternational Society for Infectious Diseases (ISID)
dc.identifier.citationNderu D, Kimani F, Thiong'o K, Akinyi M, Karanja E, Meyer CG, Velavan TP. PfHRP2-PfHRP3 diversity among Kenyan isolates and comparative evaluation of PfHRP2/pLDH malaria RDT with microscopy and nested PCR methodologies. Parasitol Int. 2018 Dec;67(6):793-799. doi: 10.1016/j.parint.2018.08.007. Epub 2018 Aug 20. PMID: 30138695.
dc.identifier.urihttps://www.sciencedirect.com/science/article/abs/pii/S138357691830268X
dc.identifier.urihttps://repository.nrf.go.ke/handle/123456789/826
dc.language.isoen
dc.publisherElsevier
dc.subjectKenya
dc.subjectMalaria
dc.subjectMicroscopy
dc.subjectPfHRP2
dc.subjectPfHRP3
dc.subjectRDTs
dc.titlePfHRP2-PfHRP3 diversity among Kenyan isolates and comparative evaluation of PfHRP2/pLDH malaria RDT with microscopy and nested PCR methodologies
dc.typeArticle
dspace.entity.typePublication

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