Publication: Antibacterial Activities Investigation of Leucas calostachys Root Extracts
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2021
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Abstract
Background and Objective: Traditional healers have long used plants to treat bacterial infections caused by resistant bacteria.
Hence, there is an increased interest in the ethnopharmacological approach to identify new novel compounds from plants to treat
these infections. The objective of this study, therefore, was to test successive extracts and their fractions. Materials and Methods:
The roots of Leucas calostachys were obtained, dried and ground. The total methanol extract was obtained and screen for antibacterial
activities. Successive extracts were extracted using four different solvents hexane, dichloromethane, ethyl acetate and methanol. The
bioactive solvent extracts were fractionated. Successive extracts and fractions were then screened for antibacterial activities against ten
pathogenic bacteria in vitro using the disk diffusion method. Results: The results indicated the solvent extracts and fractions were active
against both gram-negative and gram-positive bacteria with the lowest MIC value of 3.15 mg mLG1 obtained from total methanol extract
against methicillin-resistant Staphylococcus aureus. Conclusion: This demonstrated that hexane extracts and fractions could be helpful
in the management of resistant bacterial infections. This work is the first attempt to fractionate and test fractions of L. calostachys, which
can be used for the development of phytomedicine