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Plasmodium falciparum histidine-rich protein (PfHRP2 and 3) diversity in Western and Coastal Kenya

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dc.contributor.authorNderu, David
dc.contributor.authorKimani, Francis
dc.contributor.authorThiong’o, Kelvin
dc.contributor.authorKaranja, Evaline
dc.contributor.authorAkinyi, Maureen
dc.contributor.authorToo, Edwin
dc.contributor.authorChege, William
dc.contributor.authorNambati, Eva
dc.contributor.authorMeyer, Christian G.
dc.contributor.authorVelavan, Thirumalaisamy P.
dc.date.accessioned2024-06-20T07:02:42Z
dc.date.issued2019-02-08
dc.description.abstractPlasmodium falciparum histidine-rich proteins 2 (PfHRP2) based RDTs are advocated in falciparum malaria-endemic regions, particularly when quality microscopy is not available. However, diversity and any deletion in the pfhrp2 and pfhrp3 genes can affect the performance of PfHRP2-based RDTs. A total of 400 samples collected from uncomplicated malaria cases from Kenya were investigated for the amino acid repeat profiles in exon 2 of pfhrp2 and pfhrp3 genes. In addition, PfHRP2 levels were measured in 96 individuals with uncomplicated malaria. We observed a unique distribution pattern of amino acid repeats both in the PfHRP2 and PfHRP3. 228 PfHRP2 and 124 PfHRP3 different amino acid sequences were identified. Of this, 214 (94%) PfHRP2 and 81 (65%) PfHRP3 amino acid sequences occurred only once. Thirty-nine new PfHRP2 and 20 new PfHRP3 amino acid repeat types were identified. PfHRP2 levels were not correlated with parasitemia or the number of PfHRP2 repeat types. This study shows the variability of PfHRP2, PfHRP3 and PfHRP2 concentration among uncomplicated malaria cases. These findings will be useful to understand the performance of PfHRP2-based RDTs in Kenya.
dc.description.sponsorshipInternational Society for Infectious Diseases (ISID)
dc.identifier.citationNderu, D., Kimani, F., Thiong’o, K. et al. Plasmodium falciparum histidine-rich protein (PfHRP2 and 3) diversity in Western and Coastal Kenya. Sci Rep 9, 1709 (2019). https://doi.org/10.1038/s41598-018-38175-1
dc.identifier.urihttps://www.nature.com/articles/s41598-018-38175-1
dc.identifier.urihttps://repository.nrf.go.ke/handle/123456789/834
dc.language.isoen
dc.publisherNature Publishing Group
dc.subjectMalaria
dc.subjectMedical genetics
dc.titlePlasmodium falciparum histidine-rich protein (PfHRP2 and 3) diversity in Western and Coastal Kenya
dc.typeArticle
dspace.entity.typePublication

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