Publication:
In Vitro and In Vivo Antimalarial Activity of Nigella Sativa L. Extracts

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dc.contributor.authorOyweri Job
dc.contributor.authorMohammed Awadh
dc.contributor.authorUdu Rahma
dc.contributor.authorGathirwa Jeremiah
dc.contributor.authorToo Edwin
dc.contributor.authorOmondi Protus
dc.contributor.authorKimani Francis
dc.contributor.authorHashim Suhaila
dc.contributor.authorAbubakar Laila
dc.date.accessioned2024-07-04T08:31:54Z
dc.date.issued2019-12
dc.descriptionArticle
dc.description.abstractThe Arabs, Asians and, Traditional Health Practitioners in Mombasa county found in Kenya have been using Nigella sativa L. seeds to traditionally manage malaria associated symptoms that is, headache, fever, chills, loss of appetite among others. The present study investigated in vitro antiplasmodial, in vivo antimalarial activities and safety of different extracts of N. sativa. Five extracts obtained via aqueous extraction and sequential extraction using hexane, dichloromethane, ethyl acetate and methanol were tested against in vitro cultures of Plasmodium falciparum. The most active extracts (methanolic and ethyl acetate) were assessed for cytotoxicity and toxicity. The two active extracts were evaluated in vivo against Plasmodium berghei ANKA strain at 500, 250 and 125 mg/kg/day. On in vitro assay, methanolic and ethyl acetate extracts showed good activity with IC50 of 80.48±12.29 and 69.81±5.24 µg/ml against W2 strain and 31.93±4.31 and 53.79±6.02 µg/ml against D6 strain, respectively. The extracts exhibited weak cytotoxicity on Vero cells and high parasitemia suppression of 75.52 and 75.30% at 500 mg/kg dose of methanolic and ethyl acetate extracts respectively. Notably, there was significant decrease (p<0.001) in activity with lower doses of the extracts. The results explain thetraditional use of this plant in the Middle East and Mombasa County
dc.description.sponsorshipNational Research Fund
dc.identifier.citationOyweri, J., Mohammed, A., Udu, R., Gathirwa, J., Too, E., Omondi, P., ... & Abubakar, L. (2019). In vitro and in vivo antimalarial activity of Nigella sativa L. extracts. Journal of Medicinal Plants Research, 13(19), 501-508.
dc.identifier.urihttps://academicjournals.org/journal/JMPR/article-full-text-pdf/2FAC1D962358
dc.identifier.urihttps://repository.nrf.go.ke/handle/123456789/1090
dc.language.isoen
dc.publisherJournal of Medicinal Plants Research
dc.subjectNigella sativa L
dc.subjectseeds extracts
dc.subjectPlasmodium
dc.subjectantimalarial activity.
dc.titleIn Vitro and In Vivo Antimalarial Activity of Nigella Sativa L. Extracts
dc.typeArticle
dspace.entity.typePublication

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