Potential use of Kenyan Entomopathogenic Nematodes and Neem (Azadirachta indica) for the Sustainable Management of Tomato Leaf Miner (Tuta absoluta)
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Date
2018-06
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Abstract
The tomato leaf miner, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) was reported in
Kenya in 2014 and has become a devastating pest of tomato crop in both the field and in the
greenhouse. The objectives of this study were; to determine the pathogenicity of Heterorhabdities
species and Steinernema karii nematodes against tomato leaf miner in laboratory conditions; to
evaluate the insecticidal effects of neem biopesticide against tomato leaf miner in the greenhouse
and to evaluate the effectiveness of entomopathogenic nematodes and neem combined with
entomopathogenic nematodes as management options for tomato leaf miner in the greenhouse
conditions. Entomopathogenic nematodes (EPNs) used in the study were obtained from Kenya
Agricultural and Livestock Research Organization (KALRO) entomopathogenic nematodes
laboratories and Tuta absoluta larvae were obtained from a colony reared and maintained in a
greenhouse at Kabete Campus Field Station, Nairobi. Multiplication of the EPNs was done by invivo method or the insect-bait technique with the third instar of greater wax moth (Galleria
mellonella). Bioassays were conducted in petri dishes where the effects of EPNs concentrations;
at 100, 300and 500Ijs/ml on Tuta absoluta larvae exposed for 24-72 hours were evaluated.
Secondly, three different concentrations namely; 20ml/20L, 40ml/20L and 60ml/20L of
Nimbecidine® (Azadirachtin) were evaluated against Tuta absoluta populations in the greenhouse.
Thirdly, the following management options; Steinernema karii nematodes alone applied at a rate
of 1000 Ijs/ml with oil adjuvant Addit® as a wetting agent in water, Steinernema karii nematodes
and neem (Azadirachtin 0.03%) applied sequentially after one hour at a rate of 40ml/20L were
evaluated against Tuta absoluta populations. These were compared with Coragen® SC (20%
Chlorantraniliprole) synthetic pesticide applied at the rate of 3ml/20L and control where only water
was applied. A delta sticky trap supplied with Tuta absoluta pheromone was hanged at the centre
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of the greenhouse at a height of one metre for monitoring Tuta absoluta numbers to enable the
initiation of treatments. The results obtained showed that, the evaluated concentration rates of
Heterorhabditis species and Steinernema karii at 100Ijs/ml, 300 Ijs/ml and 500 Ijs/ml significantly
caused (p < 0.05) mortality to the Tuta absoluta larvae and the highest mortality was recorded at
500 Ijs/ml concentrations with an exposure period of 72 hours. Steinernema karii was more
pathogenic compared to Heterorhabditis species with respect to exposure time, having shown
100% and 91.5% larval mortality, respectively. Secondly, the evaluated neem concentrations
20ml/20L, 40ml/20L and 60ml/20L significantly (p < 0.05) reduced Tuta absoluta population in
the greenhouse tomato. The high concentration (60ml/20L) was more effective in reducing Tuta
absoluta population and fruit damage compared to the lower concentrations and control. High fruit
damage of 96.2% was recorded in the control compared to 30.0%, 23.4% and 20.0% for 20ml/20L,
40ml/20L and 60ml/20L dose rates, respectively. Lastly, EPNs alone and EPNs combined with
neem significantly (p < 0.05) reduced the population of Tuta absoluta. The number reduction of
Tuta absolutaby EPNs combined with neem did not differ with that which was achieved in
Coragen®. Tomato fruit damage was highest in the control with 91.5% compared to 10.8%, 7.9%
and 3.0% for EPNs alone, EPNs combined with neem and Coragen®, respectively. This study
demonstrates the potential of EPNs alone or in combination with neem applied successively at a
one hour interval, as an alternative strategy for the sustainable management of Tuta absoluta in
the greenhouse conditions.