Animal Breeding

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Browsing Animal Breeding by Funder "Bill and Melinda Gates Foundation, Non US Government"

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    Publication
    Distribution of Rotavirus Genotypes among Children with Diarrhea Prior to Vaccine Introduction in Western Kenya
    (Journal of Infectious Diseases and Epidemiology, 2019-02-16) Ochieng, John B; Khagayi, Sammy; Ogwel, Billy; Onkoba, Reuben; Apondi, Evans; Juma, Jane; Seheri, Mapaseka; Peenze, Ina; Onyango, Clayton; Hunsperger, Elizabeth; Tabu, Collins; Kibet, Sergon; Omore, Richard; Odhiambo, Frank; Mphahlele, Jeffrey; Verani, Jennifer R; Fields, Barry; Bigogo, Godfrey; Laserson, Kayla F; Tate, Jacqueline E; Mwenda, Jason M; Breiman, Robert F
    Background: Group A rotavirus is the leading cause of diarrhea hospitalization among children worldwide. Most fatal rotavirus-associated diarrhea cases among children under 5 years occur in Africa and Asia. We investigated the molecular epidemiology of circulating rotavirus strains among children less than 5 years in western Kenya to provide baseline data on the prevalence of each genotype prior to the introduction of monovalent rotavirus vaccine in Kenya. Methods: From January 2010 to December 2013, stool samples were collected from 1677 children with acute gastroenteritis. All specimens were transported to Kenya Medical Research Institute, Center for Global Health Research, Enterics Laboratory and tested for rotavirus antigens using enzyme immunoassay. Rotavirus dsRNA was extracted from 234 simple randomly selected EIA positive stool samples using QIAamp viral RNA mini kit and tested by semi-nested RT-PCR for G and P genotypes using type-specific primers. The PCR products were analyzed by gel electrophoresis. Chi-square test was used to test the association between rotavirus genotypes and age. Results: Of the 1677 stool samples tested, 401 (23.9%) were positive for group A rotavirus antigen. Of the 234 rotavirus dsRNA extractions analyzed by PCR, 219 (93.6%) and 193 (82.5%) typed positive for at least one of the VP7 genotypes (G type) and VP4 genotypes (P types), respectively. Of the typeable, 19 were mixed G types and P types. However, 15 VP7 and 41 VP4 were nontypeable. The predominant genotypes detected included G1 (30%), G9 (27%), G8 (10%) and G3 (9%) for the G types, and P[8] (33%) and P[6] (30%) for the P types. The predominant combinations were: G1P[8] (15%), G9P[8] (12%) and G3P[6] (8%) which combined accounted for 35% of the genotypes detected. Conclusion: This study demonstrated the genotype diversity and dominance of G1, G3, G8 and G9 in combination with P[6] and P[8] as the most common genotypes associated with rotavirus gastroenteritis in this population. Continuous surveillance is necessary to monitor the effectiveness of the vaccine and shifts among the circulating genotypes in this region.
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    Publication
    Prevalence and Fluid Management of Dehydration in Children without Diarrhoea Admitted to Kenyan Hospitals: A Multisite Observational Study
    (BMJ Open, 2021-03-17) Omoke, Sylvia; English, Mike; Aluvaala, Jalemba; Gathara, David; Agweyu, Ambrose; Akech, Samuel
    Objectives To examine the prevalence of dehydration without diarrhoea among admitted children aged 1–59 months and to describe fluid management practices in such cases. Design A multisite observational study that used routine in-patient data collected prospectively between October 2013 and December 2018. Settings Study conducted in 13 county referral hospitals in Kenya. Participants Children aged 1–59 months with admission or discharge diagnosis of dehydration but had no diarrhoea as a symptom or diagnosis. Children aged <28 days and those with severe acute malnutrition were excluded. Results The prevalence of dehydration in children without diarrhoea was 3.0% (2019/68 204) and comprised 15.9% (2019/12 702) of all dehydration cases. Only 55.8% (1127/2019) of affected children received either oral or intravenous fluid therapy. Where fluid treatment was given, the volumes, type of fluid, duration of fluid therapy and route of administration were similar to those used in the treatment of dehydration secondary to diarrhoea. Pneumonia (1021/2019, 50.6%) and malaria (715/2019, 35.4%) were the two most common comorbid diagnoses. Overall case fatality in the study population was 12.9% (260/2019). Conclusion Sixteen per cent of children hospitalised with dehydration do not have diarrhoea but other common illnesses. Two-fifths do not receive fluid therapy; a regimen similar to that used in diarrhoeal cases is used in cases where fluid is administered. Efforts to promote compliance with guidance in routine clinical settings should recognise special circumstances where guidelines do not apply, and further studies on appropriate management for dehydration in the absence of diarrhoea are required.