Publication: Enzymatic activity and brine shrimp lethality of venom from the large brown spitting cobra (Naja ashei) and its neutralization by antivenom
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2020
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Funder
NRF
Publisher
BMC Research Notes
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Abstract
Naja ashei is a snake of medical importance in Kenya, Ethiopia, Somalia, Uganda, and Tanzania. Little
is known about the enzymatic (snake venom phospholipases A2; svPLA2’s) and toxic (lethal) activities of N. ashei
venom and crucially, the safety and capacity of available antivenom to neutralize these effects. This study aimed to
determine the enzymatic and toxic activities of N. ashei venom and the capacity of Indian and Mexican manufactured
antivenoms to neutralize these effects. The protein content of the venom and the test antivenoms were also
evaluated. A 12-point log concentration–response curve (0.5–22.5 µg/mL) was generated on an agarose-egg yolk
model to predict the svPLA2 activity of the venom. The toxicity profiles of the venom and antivenoms were evaluated
in the brine shrimp lethality assay. Lowry’s method was used for protein estimation.
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Keywords
Snake venom phospholipases A2, Brine shrimp lethality assay, Snake venom toxicity, Naja ashei, Brine shrimp, Artemia salina, Probit analysis, LC50, EC50, MPC50