FISH PROTEIN HYDROLYSATE FROM DAGAA (Rastrineobola argentea) AS NUTRITIONAL COMPONENT IN MICROBIAL CULTURE MEDIA
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Date
2016-09
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Abstract
Dagaa is a small pelagic fish found in Lake Victoria. It is mainly used as a nutrient
source in the animal feed industry as well as for human consumption among the
poor and the middle class. Fish protein hydrolysate as a source of nutrient in culture
media has been explored in several fish species such as Silver Carp, Yellow Tuna,
Yellow Stripe Trevally, Herring and Mackerel. Dagaa could also be an excellent
candidate for culture media since it is rich in proteins, lipids and minerals. This
would also add value to the underutilized fish species and minimize post-harvest
losses of up to 50% during rainy seasons. In the current study, Dagaa as a source of
nitrogen in culture media was explored by first preparing the Dagaa Protein
Hydrolysate (DPH) using Alcalase enzyme. Proximate analysis for protein, lipid,
moisture /dry weight and ash contents of DPH was performed and compared with
that of Dagaa, Dagaa Fish Hydrolysate (DFH), Standard media Luria Bertani (LB)
and Sabouraud’s Dextrose Broth (SDB). Mineral analysis was also performed on all
these samples. The potential of DPH as a nitrogen source in microbial growth media
was investigated using; Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis
and Rhodobacter capsulatus for bacterial growth studies while Metarhizium
anisopliae and Beauveria bassiana were used for fungal growth. Potential of DPH
to sustain production of commercial valued enzyme amylase was also investigated
with R. capsulatus and B. subtilis in DPH-Starch media. This was done using
Dinitrosalicylic (DNS) acid assay. M. anisopliae was analyzed for sporulation
(conidia production) rate in DPH-Dextrose media. Proximate and mineral analysis
indicated that there was significant difference (p<0.05) in the nutritional and mineral
profiles of DPH and the two standard media (LB and SD). The protein content of
DPH was higher than that of LB and SDB while the mineral content of DPH was
lower than that of LB but slightly higher than that of SDB. LB had more Sodium
content than DPH. Investigation of the potential of DPH as a nitrogen source in
media indicated that in all microorganisms used in this study, there was no
significant difference (p˃0.05) in DPH-FM and DPH-Dextrose as compared to
standard media in the growth of microorganisms. The potential for DPH-Starch to
sustain production of commercial valued amylase indicated that there was
significant difference (p<0.05) in the sustenance of amylase production in
formulated DPH-Starch and standard media by R. capsulatus and B. subtilis. DPHStarch media gave better production of amylase enzyme in both microbes than the
standard media. There was no significant difference (p˃0.05) in the sporulation rates
of M. anisopliae in DPH-Dextrose and the standard media. Hence DPH formulated
media can be substituted as a potential culture media for microbial growth. The cost
of formulation is low and provides an alternative means of value addition to Dagaa.